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Delivering confidence in your microscope images 

PSFcheck calibration slides offer a quick and easy way to:

  • Quantify imaging performance 

  • Track changes in image quality

  • Support your research findings 

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Over 150 PSFcheck slides sold worldwide

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Journal papers citing PSFcheck

1. Jeremy Metz, Michele Gintoli, Alexander D. Corbett, "Fully automated point spread function analysis using PyCalibrate"Biol Open 12 (11): bio059758 (2023)

2. Zhengyi Lu, Gregory Cross, Yu-Chieh Yuan, Alfredo Cardenas-Rivera, Chang Liu, Mohammad A Yaseen, "Custom cannula for in vivo subcortical two-photon microscopy in mouse models of Alzheimer’s disease",
Neural Imaging and Sensing 2023 12365, 51-57, (2023).

3. Sharika Mohanan and Alexander D. Corbett, "Understanding the limits of remote focusing," Opt. Express 31, 16281-16294 (2023).

4. Esley Torres García, Raúl Pinto Cámara, Alejandro Linares, Damián Martínez, Víctor Abonza, Eduardo Brito-Alarcón et. al. "Nanoscopic resolution within a single imaging frame", (2022) bioRxiv 2021.10.17.464398;

5. Torres-García, E., Pinto-Cámara, R., Linares, A. et al. "Extending resolution within a single imaging frame." Nat Commun 13, 7452 (2022).
6. Kopanchuk S, Vavers E, Veiksina S, Ligi K, Zvejniece L, et al. (2022) "Intracellular dynamics of the Sigma-1 receptor observed with super-resolution imaging microscopy"PLOS ONE 17(5): e0268563.

7. McCluskey, K. A. et al. “Global correction of optical distortions in multicolor single-molecule microscopy using Zernike polynomial gradients”. Opt. Express 29, 42251 (2021).

8. Hurley, M. E. et al. “A correlative super-resolution protocol to visualise structural underpinnings of fast second-messenger signalling in primary cell types”. Methods 193, 27–37 (2021).
9. Michele Gintoli, Sharika Mohanan, Patrick Salter, Elizabeth Williams, James D. Beard, Gaspar Jekely, and Alexander D. Corbett, "Spinning disk-remote focusing microscopy," Biomed. Opt. Express 11, 2874-2888 (2020).

10. Alexander D. Corbett, Michael Shaw, Andrew Yacoot, Andrew Jefferson, Lothar Schermelleh, Tony Wilson, Martin Booth, and Patrick S. Salter, "Microscope calibration using laser written fluorescence," Opt. Express 26, 21887-21899 (2018).

The PSFcheck slide

The PSFcheck slide uses standard microscope slide dimensions to make imaging even easier

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Male Scientist


Jeremy Graham

CEO, Cairn Research Ltd.

“Based on our observations so far we expect the PSFcheck slides to be a very useful microscopy tool”

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